MersV1, Main, Exploration, bibRecord, 001043

Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis)

Identifieur interne : 001043 ( Main/Exploration ); précédent : 001042; suivant : 001044

Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis)

Auteurs : Vincent J. Munster ; Danielle R. Adney ; Neeltje Van Doremalen ; Vienna R. Brown ; Kerri L. Miazgowicz ; Shauna Milne-Price ; Trenton Bushmaker ; Rebecca Rosenke ; Dana Scott ; Ann Hawkinson ; Emmie De Wit ; Tony Schountz ; Richard A. Bowen

Source :

Scientific Reports [ 2045-2322 ] ; 2016.

RBID : PMC:4761889

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (sang), Cellules Vero, Charge virale, Chiroptera (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Cricetinae, Dipeptidyl peptidase 4 (métabolisme), Excrétion virale, Immunité innée, Infections à coronavirus (immunologie), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (sang), Infections à coronavirus (virologie), Poumon (anatomopathologie), Poumon (virologie), Récepteurs viraux (métabolisme), Réplication virale.
MESH :
- anatomopathologie : Poumon.
- immunologie : Infections à coronavirus.
- médecine vétérinaire : Infections à coronavirus.
- métabolisme : Dipeptidyl peptidase 4, Récepteurs viraux.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- sang : Anticorps antiviraux, Infections à coronavirus.
- virologie : Chiroptera, Infections à coronavirus, Poumon.
- Animaux, Cellules Vero, Charge virale, Cricetinae, Excrétion virale, Immunité innée, Réplication virale.

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), Chiroptera (virology), Chlorocebus aethiops, Coronavirus Infections (blood), Coronavirus Infections (immunology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Cricetinae, Dipeptidyl Peptidase 4 (metabolism), Immunity, Innate, Lung (pathology), Lung (virology), Middle East Respiratory Syndrome Coronavirus (physiology), Receptors, Virus (metabolism), Vero Cells, Viral Load, Virus Replication, Virus Shedding.
MESH :
- chemical , blood : Antibodies, Viral.
- blood : Coronavirus Infections.
- immunology : Coronavirus Infections.
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- pathology : Lung.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- veterinary : Coronavirus Infections.
- virology : Chiroptera, Coronavirus Infections, Lung.
- Animals, Chlorocebus aethiops, Cricetinae, Immunity, Innate, Vero Cells, Viral Load, Virus Replication, Virus Shedding.

Abstract

The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the zoonotic potential of Betacoronaviruses. Investigations into the origin of MERS-CoV have focused on two potential reservoirs: bats and camels. Here, we investigated the role of bats as a potential reservoir for MERS-CoV. In vitro, the MERS-CoV spike glycoprotein interacted with Jamaican fruit bat (Artibeus jamaicensis) dipeptidyl peptidase 4 (DPP4) receptor and MERS-CoV replicated efficiently in Jamaican fruit bat cells, suggesting there is no restriction at the receptor or cellular level for MERS-CoV. To shed light on the intrinsic host-virus relationship, we inoculated 10 Jamaican fruit bats with MERS-CoV. Although all bats showed evidence of infection, none of the bats showed clinical signs of disease. Virus shedding was detected in the respiratory and intestinal tract for up to 9 days. MERS-CoV replicated transiently in the respiratory and, to a lesser extent, the intestinal tracts and internal organs; with limited histopathological changes observed only in the lungs. Analysis of the innate gene expression in the lungs showed a moderate, transient induction of expression. Our results indicate that MERS-CoV maintains the ability to replicate in bats without clinical signs of disease, supporting the general hypothesis of bats as ancestral reservoirs for MERS-CoV.

Electronic supplementary material

The online version of this article (doi:10.1038/srep21878) contains supplementary material, which is available to authorized users.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761889

DOI: 10.1038/srep21878
PubMed: 26899616
PubMed Central: 4761889

Affiliations:

Le document en format XML

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<term>Antibodies, Viral (blood)</term>
<term>Chiroptera (virology)</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus Infections (blood)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Coronavirus Infections (virology)</term>
<term>Cricetinae</term>
<term>Dipeptidyl Peptidase 4 (metabolism)</term>
<term>Immunity, Innate</term>
<term>Lung (pathology)</term>
<term>Lung (virology)</term>
<term>Middle East Respiratory Syndrome Coronavirus (physiology)</term>
<term>Receptors, Virus (metabolism)</term>
<term>Vero Cells</term>
<term>Viral Load</term>
<term>Virus Replication</term>
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<front><div type="abstract" xml:lang="en"><p>The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the zoonotic potential of <italic>Betacoronaviruses</italic>
. Investigations into the origin of MERS-CoV have focused on two potential reservoirs: bats and camels. Here, we investigated the role of bats as a potential reservoir for MERS-CoV. <italic>In vitro</italic>
, the MERS-CoV spike glycoprotein interacted with Jamaican fruit bat (<italic>Artibeus jamaicensis</italic>
) dipeptidyl peptidase 4 (DPP4) receptor and MERS-CoV replicated efficiently in Jamaican fruit bat cells, suggesting there is no restriction at the receptor or cellular level for MERS-CoV. To shed light on the intrinsic host-virus relationship, we inoculated 10 Jamaican fruit bats with MERS-CoV. Although all bats showed evidence of infection, none of the bats showed clinical signs of disease. Virus shedding was detected in the respiratory and intestinal tract for up to 9 days. MERS-CoV replicated transiently in the respiratory and, to a lesser extent, the intestinal tracts and internal organs; with limited histopathological changes observed only in the lungs. Analysis of the <italic>innate</italic>
 gene expression in the lungs showed a moderate, transient induction of expression. Our results indicate that MERS-CoV maintains the ability to replicate in bats without clinical signs of disease, supporting the general hypothesis of bats as ancestral reservoirs for MERS-CoV.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1038/srep21878) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
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Serveur d'exploration MERS

Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis)

Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis)

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